Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGHTFKLPVVPANMQTIIDEKLAISLAENGYFYVMHRF----EPETRIDFIKDMNARGLFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASK---PIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEYFGSAS----EFQKGEKKNV-EGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAIRNVDYVIVKNSIFNGDKY 4QM1 Chain:C ((35-358)) ----LTFDDVLLVPAKSDVLPREVSVKTVLSESLQLNIPLISAGMDTVTEADMAIAMARQGGLGIIHKNMSIEQQAEQVDKVK--RSGGLLVGAAVGVTADAMTRIDALVKASV--DAIVLDTAHGHSQGVIDKVKEVRAKYPSLNIIAGNVATAEATKALIEAGANVVKVGIGPGSICTTRVVAGVGV--PQLTAVYDCATEARKHGIPVIADGGIKYSGDMVKALAAGAHVVMLGSMFAGVAESPGETEIYQGRQFKVYRGMGSVGAM---------VPEGIEGRVPYKGPLADTVHQLVGGLRAGMGYCGAQDLEFLR-----------------

General information: TITO was launched using: RESULT: Template: 4QM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1757 -40460 -23.03 -139.52 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -23.03 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4QM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QM1-query.scw PDB file : Tito_Scwrl_4QM1.pdb: