Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMAKSYLITGGAGFIGLTFTKLMLRETD--ARITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDE-TYDGVIHFAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDLKADDPAFTETTPLSPNNPYSASKASSDLLVLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVYNIGGGNERTNKELASVILKHLGCEE-LFAHVEDRKGHDRRYAINASKLKNELGWRQEVTFEEGIARTIQWYTDNDR
4EGB Chain:A ((24-338))-AMNILVTGGAGFIGSNFVHYMLQS-YETYKIINFDALTYSGNLNNVKSIQDHPNYYFVKGEIQNGELLEHVIKERDVQVIVNFAAES---------IPFYDTNVIGTVTLLELVKKYPHIKLVQVSTDEVYGSLGKT-GRFTEETPLAPNSPYSSSKASADMIALAYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWLHVTDHCSAIDVVLHKGRVGEVYNIGGNNEKTNVEVVEQIITLLGKTKKDIEYVTD--GHDRRYAINAEKMKNEFDWEPKYTFEQGLQETVQWYEKNE-


General information:
TITO was launched using:
RESULT:

Template: 4EGB.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1709 20650 12.08 68.83
target 2D structure prediction score : 0.68
Monomeric hydrophicity matching model chain A : 0.84

3D Compatibility (PKB) : 12.08
2D Compatibility (Sec. Struct. Predict.) : 0.68
1D Compatibility (Hydrophobicity) : 0.84
QMean score : 0.561

(partial model without unconserved sides chains):
PDB file : Tito_4EGB.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4EGB-query.scw
PDB file : Tito_Scwrl_4EGB.pdb: