Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQTIRVELTSTKKPKPDPNQ-LSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIILSPVGSYYKEG-IKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIFFKING-----EIVTPMLNGSILEGITRNSVIALLKHWG-LQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLSINNGETG-EIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK 5I5T Chain:B ((14-365)) -----LQLEMTQKPHKKPGP--PLVFGKTFTDHMLMVEWN-DKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGA-----SVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQ-LTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEW-------------

General information: TITO was launched using: RESULT: Template: 5I5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1770 -55421 -31.31 -164.94 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -31.31 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_5I5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I5T-query.scw PDB file : Tito_Scwrl_5I5T.pdb: