Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAAYQARCQARVDAALDALFVAPREELQRLYEAMRYSVMNGGKRVRPLLAYAACEALGGAPQRAD-AAACAVELIHAYSLVHDDLPAMDDDDLRRGQPTTHRAFDEATAILAADGLQALAFEVLADTRRNPQEHAVCLEMLTRLARAAGSAGMVGGQAIDLGSVGVALDQAALEVMHRHKTGALIEASVRLGALASGRAEPASLAALERYAEAIGLAFQVQDDILDVESDTATLGKTQGKDQAHNKPTYPALLGLEAAKGYALELRDLALAALDGFPPSADPLRQLARYIVERRN 4LFG Chain:A ((7-250)) -----------KIDQTIHAFYCEKAVISEKLNEAVLYSINAGGKRIRPILFLEVIEALQIPLTESHFKAAAALEMIHTGSLIHDDLPAMDNDDYRRGQLTNHKKFDEATAILAGDSLFLDAFGMLAETDF-PTD--VTVDLVRSLSSASGTFGMVGGQMLDMAAEGKKLNLKNLQLIHRHKTGQLL-AYPFWAAARVAQLDENLLATFLEIGMIIGLAFQVRDDILDITANFEEIGKTPKKDVMAEKMTYPHLLGLNES-------------------------------------

General information: TITO was launched using: RESULT: Template: 4LFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -58507 for 1964 contacts (-29.8/contact) + 2D Compatibility (PS) -27369 + (NN) -27524 + (LL) 4288 1D Compatibility (HY) -20000 + (ID) 5400 Total energy: -134512.0 ( -68.49 by residue) QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_4LFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFG-query.scw PDB file : Tito_Scwrl_4LFG.pdb: