Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQHETPLYTGLKKHASRQPVQFHIPGHKKGAGMDPEFRQFIGENALSIDLINIEPLDDLHAPKGIIKQAQDLAAEAFGAD--HTFFSVQGTSGAIMTMVMAVC----GPGDKIIIPRNVHKSIMTA----IVFSGAVPIFIHPEIDNELGISHGITLESAKRALTEHPDAKGLLVINP-TYFGVAADLKSIVELAHSFDVPVLVDEAHGV-HIHFHDELPLSAMQAGADIAATSVHKLGGSLTQSSILNMREGLVSKDRVQSI--------------------LSMLTTTSTSYLLLASLDVARKRLATEG-RQLAEETLKLANQTRDRLNQIEGIYCVGSEILGSKAAYSYDPTKLIISVKSLGLTGHDVEKWLRESFNIEVELSD------------LYNILCIFTPGDSQNDADRLVEALTEIAQQMSEQDVTHQQTEVLLPEIPLLAMTPRDAFYANTEVIPLKEASGRIIAEFVMVYPPGIPIFIPGEIITEENISYIFKNLDAGLPVQGPEDSTLHMIRVIKEQKAIQ 4LW2 Chain:A ((65-402)) ---------------------------------------------------------------TARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQ------RLPDVDLLPELIT--PRSRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADV------QQLDIDFYAFSGHKLY-GPTGIGVLYGKSELL--EAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQ----------DSSLLAFDFA--GVHHSDMVTLLAE-YGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELL--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LW2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1586 -213058 -134.34 -727.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -134.34 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_4LW2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LW2-query.scw PDB file : Tito_Scwrl_4LW2.pdb: