Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRKPDEIAEAAIEAGMKKIKLPLPS---LLVLGFLGGAFIALGYLLDIRVIGDLPKEWGS-LSSLIGAAVFPVGLILVVLAGAELITGNMMSVAMALFSRKISVKELAINWGIVTIMNLIGALFVAYFFGHLVGLTETGPYLEKTIAVAQGKLDMSFGKVLISAIGCNWLVCLAVWLSFGAQDAAGKILGIWFPIMAFVAIGFQHVVANMFVIPAAIFA---GSFTWGQFIGNIIPAFIGNVIGGAVFVGLIYFIAYHKKDRSRKEMKQVS 3TE1 Chain:C ((3-265)) ---RAHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL-IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFAGNNMVMSAGMLNKGVSIKDTSKIWAYSWVGNLIGALVLGIIFVG-TGLVDKGPVAEFFANTAASKASMPFTALFFRGILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTISTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLNAAAE-----

General information: TITO was launched using: RESULT: Template: 3TE1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1411 -189456 -134.27 -740.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -134.27 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_3TE1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TE1-query.scw PDB file : Tito_Scwrl_3TE1.pdb: