Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEADMLFSVTVPGSTANLG-----PGFDSVGMALSRYLKLTVFESDKWSFEAET-E----TV-AGIP--AGTDNLIYQVAKRTADLYGKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEEG--------HPDNAGASLVG---GLVIGLHEDDETQMIRVPN-ADIDVVVVIPFYEVLTRD-ARDV-----------LPKEF---------P-Y--------ADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRA---MLVPELSKVEHVAEMKGAYGTALSGAG--PTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV 2CZ9 Chain:A ((1-327)) ------MIKVKSPGRVNLIGEHTDYTYGYVMPMAINLYTKIEAEKHGEVILYSEHFGEERKFSLNDLRKENSWIDYVKGIFWVLKE-SDYEVGGIKGRVSGNLPLGAGLSSSASFEVGILETLDKLYNLKLDSLSKVLLAKKAENEFVGVPCGILDQF-AVVFGREGNVIFLDTHT--LDYEYIPFPKDVSILVFYTGVR----SSEYAERKHIAEESLKILGKGSSKEVREGELSKLPPLHRKFFGYIVRENARVLEVRDALKEGNVEEVGKIL-TT-AHWDLAKNYEVSCKELDFFVERALKLGAYGARLTGAGFGGSAIALVDKEDAETIGEEILR------------------------------

General information: TITO was launched using: RESULT: Template: 2CZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1345 9230 6.86 35.09 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 6.86 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_2CZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CZ9-query.scw PDB file : Tito_Scwrl_2CZ9.pdb: