Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKTISGAERLSLKRSIDAGTEEQRKTVRSIIEDVKANGDQAVRSYTAKFDCIEIDSPLVTKEEFEEAYTSLDSRLLQVIRQAIENIREYHERQLQSSWFYHRKDGTMLGQKVTALDSAGVYVPGGTAAYPSSVLMNVIPALVAGVERIVLVTPPGKDGLLSPGVLVAAAELGIKDIYKMGGAQAIAALAYGTETIEPVDKITGPGNIYVALAKREVFGDVDIDMIAGPSEIVVLADETAIPSEIAADLLSQAEHDKLSSCVFVTDSMALAETVSAEVNKQLETLPRREIAEASVRDYGCIYVAETMVEAIETVNTLAPEHLEIITQSPEALLGSIKHAGAIFLGRYSPEPVGDYFAGPNHVLPTNGTARFSSPLNVTDFQKKSSIISYSQSAFEEHAESIAAFARLEGLEAHARSIEARERRISK 4O6M Chain:A ((29-370)) MRLAYVKNHEIYG--------EKLLGLTLRERIEKTLQRAGFDVRFFDE-----------LSLEEAEDYLIILEPVLILERDLLLEGRKILVSDGFTVGYFFG-------------GDFRTVFDGNLQSSIEKYLSLNNLESYEIWAIK------------LSNDNLKTAEKLLLSSLIDGWIAREI--------------------NRKVSLRISRLLADTSVT----PNQITVFSFFLSL---VGSALFLLNSYLTTLLAGVIIQLHSIIDGCDGEIARLKFMESKYGAWLDGVLDRYSDFIIVFSITYVLSASN--PVYWIIGFLAAFASL-MIAYTGDKFVAAYMRTYSPEGFAIPI----------------TRDFRL---LIIFACSVVNLPSLALVIIALLGNFEALRRIVALRSY----

General information: TITO was launched using: RESULT: Template: 4O6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1566 -132054 -84.33 -400.16 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -84.33 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_4O6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6M-query.scw PDB file : Tito_Scwrl_4O6M.pdb: