Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDA-----HKLYLVFEFLDLDLKRYMEGIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVY--LPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 2FST Chain:X ((28-317)) ----PERYQNLSPVG----GSVCAAFDTKTG---LRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLN---------QKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILD------------------ATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQ-

General information: TITO was launched using: RESULT: Template: 2FST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -154516 for 1918 contacts (-80.6/contact) + 2D Compatibility (PS) -27267 + (NN) -6324 + (LL) 2064 1D Compatibility (HY) -26000 + (ID) 4800 Total energy: -216843.0 ( -113.06 by residue) QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_2FST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FST-query.scw PDB file : Tito_Scwrl_2FST.pdb: