Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITKEEVNSYYQKAGIVLTDEEVDQIQLMDYGLGKERKVGLQLFVYVNTDRYCSKELVLFPGQTCPEHRHPPVDGQEGKQETFRCRYGKVYLYVE-GEKTPLPKVLPPQEDREHYTVWHEIELEPGGQYTIPPNTKHWFQAGE-EGAVVTEMSSTSTDKHDIFTDPRI 3H8U Chain:A ((31-121)) --------------------------------------------SVVLETNDSVVVVWHAHPGQEIASHVHP------HGQDTWTVISGEAEYHQGNGIV---------------------THLKAGDIAIAKPGQVHGAMNSGPEPFIFVSVVAPGNAGFA-------

General information: TITO was launched using: RESULT: Template: 3H8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 420 -28948 -68.92 -325.26 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -68.92 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.733

(partial model without unconserved sides chains): PDB file : Tito_3H8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H8U-query.scw PDB file : Tito_Scwrl_3H8U.pdb: