Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRYRTLLFDVDDTILDFQAAEALALRLLFEDQNIPLTND-MKAQYKTINQGLWRAFEEGKMTRDEVVNTRFSALLKEYGYEAD-GALLEQKYRRFLEEGHQLIDGAFDLISNLQ-QQFDLYIVTNGVSHTQYKRLRDSGLFPFFKDIFVSEDTGFQKPMKEYFNYVFERIPQFSAEHTLIIGDSLTADIKGGQLAGLDTCWMNPDMKPNVPEIIPTYEIRKLEELYHILNIENTVSC 2GO7 Chain:A ((4-206)) ---KTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREFIFK-------------YSVQD----LLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQQFIYTHKGN-NAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKY-QLNSDNTYYIGDRT-LDVEFAQNSGIQSINFLESTY------EGNHRIQALADISRIFET------

General information: TITO was launched using: RESULT: Template: 2GO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 993 -120171 -121.02 -600.85 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -121.02 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2GO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GO7-query.scw PDB file : Tito_Scwrl_2GO7.pdb: