Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKL---EDQVDEVILGNA--TGRGGNLARLSALQAGLPLSVPGMTIDRQCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSERAR-------------FSPDAIGDP----DMGIAAEYTAARYSISRSMQDEYALLSHQRSRNAHDEGFYREEVVAL------GELETD-EAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLKPVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQELGIPFSKINVRGGALALGHPYGASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLFEVLA 2VU2 Chain:C ((6-392)) ---VIASAARTAVGSFNGAFANTPAHELGATVISAVLERAGVAAGEVNEVILGQVLPAGEGQNPARQAAMKAGVPQEATAWGMNQLCGSGLRAVALGMQQIATGDASIIVAGGMESMSMAPHCAHLRGGVKMGDFKMIDTMIKDGLTDAFYGYHMGTTAENVAKQWQLSRDEQDAFAVASQNKAEAAQKDGRFKDEIVPFIVKGRKGDITVDADEYIRHGATLDSMAKLRPAFD-KEGTVTAGNASGLNDGAAAALLMSEAEASRRGIQPLGRIVSWATVGVDPKVMGTGPIPASRKALERAGWKIGDLDLVEANEAFAAQACAVNKDLGWDPSIVNVNGGAIAIGHPIGASGARILNTLLFEMKRR-GARKGLATLCIGGGMGVAMCIESL-

General information: TITO was launched using: RESULT: Template: 2VU2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2545 -209856 -82.46 -586.19 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -82.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2VU2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VU2-query.scw PDB file : Tito_Scwrl_2VU2.pdb: