Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKQKKKLCQLQLNEIKTSDDPTKLSCSFVIFDFDVSHNNTVISKDVALEAASTIINKPIVAKYYEVDELNTSTDALGTHEAYLDTDKHGELEVKRDTAPIGVFTSEGYITEIETPDGKKEVLAADAILWSSRFKDACELLLEWYGRGININTSCEILYSNYTVQDGIEHLQSPIYFEGHAILNSEKRGEHDIVLPAYDSSKLLSFNELQRFERLVAQAATRQNNEEGEKMNKFRKVFELSHSDVRTLLYSQLDPTLDKESDSFIADVYD----TYFIVNVYSWSDENSYDKYFKFNYTRTGDTVSIDFDSKTEVFMTRNWEEVVPEPIQSQLNQKDEQIKDLTKQVNQINKDKVGIEQQFNTASEKLVQLNSEVEQLKP-------YKEKHEKTLLEQKLSE----KNEFYKAKFEALNAEEKFSTEEVQNLIHASVKQDEEGEKAVLQLNTMLVDLVSVPTETNTTIREFSSKRENLIPNDDSFESRFSQ--------- 4YLA Chain:A ((2-375)) -------------------AGDPFV--------------DNGTVSSQRPLRAVPGRYPPGATHLDAAVDTLVRCHAALG----------RAPSEAEAAVCLLRRLWGRWGNTPVERP-GWRSYVAVDGS--------PFELSAAWNGDG-----PAEVRVTVEATADP----PTPEGNQEAGWEYLRGLSRH----PGAATARVLALEDLFRPQTPHDRCW------------IMHGMASRPGADPLFKVYLDPDARGAAEAPSVLDEAMDRLGVRAAWQGLRGWLDEHG----------GSGRIGSLALD----------LADTDDARVKVYVQHAGLDWADIDRQAAVARGHVPG---AFSAALEEIT--GTEVPPHKPPVTCFAFHRGVGVPTAATLYIPMPAGVPESDARRRSAAFMRRSGLDSAAYLAFLAAATG-DGEGVRA-------LQNFVAYR------------------PAAPGGRPRFACYVAPGLYRL

General information: TITO was launched using: RESULT: Template: 4YLA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1858 18818 10.13 53.77 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 10.13 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_4YLA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YLA-query.scw PDB file : Tito_Scwrl_4YLA.pdb: