Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGFFDSGIGGMTVLYEAIKVLPYEDYIFYADTLNVPYGEKSKGKVKEYIFNAAEFLASQNIKALVIACNTATSIAIEDLRRNFDFPIIG-IEPAVKPAINKCTEERKRVLVVATNLTLKEEKFHNLVKEIDHHDLVDCLALPGLVEFAENFDFSEDKIIKYLKNELSSFDLKQYGTIVLGCTHFPFFKNSFEKLFGIKVDMISGSVGTAKQLKKVLADRNQLGKGSGSITFFNSGHKIVDQEVISKYKRLFEILDETQRSHVGH 1B74 Chain:A ((1-252)) MKIGIFDSGVGGLTVLKAIRNRYRKVDIVYLGDTARVPYGIRSKDTIIRYSLECAGFLKDKGVDIIVVACNTASAYALERLKKEINVPVFGVIEPGVKEALKKS--RNKKIGVIGTPATVKSGAYQRKLEE--GGADVFAKACPLFAPLAEEGLLEGEITRKVVEHYLKEFK-GKIDTLILGCTHYPLLKKEIKKFLG-DAEVVDSSEALSLSLHNFIKD-----DGSSSLELFFTDL---SPNLQFLIKLILGRDYPVKLAEGVF

General information: TITO was launched using: RESULT: Template: 1B74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1386 -89573 -64.63 -356.86 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -64.63 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_1B74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B74-query.scw PDB file : Tito_Scwrl_1B74.pdb: