Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQLIQSEEEFKRIAEQEGVFVFLKHSTTCPISQAAFHEFDAFANQHEDVPAYYLQVQEARPLSNFIAETYGVKHESPQIFIIQNGEVKWHTSHSQITEAAIEQHLS 4CW9 Chain:A ((2-99)) -AVLHINALDQLTALLSTEKVIVIDFFATWCGPSRSISPYFEELAGQYNNIKFVKVDVDQAEE----ICVNYKVRS-MPTFVLVKDGIEQKRFS--GADRNALKQM--

General information: TITO was launched using: RESULT: Template: 4CW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 416 -69815 -167.82 -712.39 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -167.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.706

(partial model without unconserved sides chains): PDB file : Tito_4CW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CW9-query.scw PDB file : Tito_Scwrl_4CW9.pdb: