Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILH-RFEDAVIEVQELKEEVAGTLAVGSTIYCAALMLEKV-TQIKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDTVQFKQL-DDIPCVLVLSDEAGYPCGDTIKMVDIPSFP-LILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATL-LSL-VSSGFGASILP-VTMIPVHMRNHVHT-VHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF------ 3FZV Chain:A ((4-300)) YTLRQLKYFVTTVECGSVAEASRKLYI-AQSISTAVKGLEESFV----------QLFLTPAGARFYRKAQELLRMAHEFEQNLADN--DVIAGQIDIGCFETVAPLYLPGLIAGFRQAYPGVEIRIRDGEQQELVQGLTSGRFDLAFLYEHDLDSTIETEPLMPPQRPHALLPEGHRFAGQAQVSLRDLCLEPMILLDVQ----PSRTYFVSLFEELGLTPNI--AFSSPSIEMVRGMVGQGFGFSLLVTRPHSECTYDGKKVVMVDLAEPVSTSGLAAAWLKRAQLTKPARLFVDYCREQLGK

General information: TITO was launched using: RESULT: Template: 3FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1212 -176354 -145.51 -650.75 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -145.51 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_3FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZV-query.scw PDB file : Tito_Scwrl_3FZV.pdb: