Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKRITFG-LLVLMVCVILFVLYVQLRNKDSIPVQS-SAIHPEEDRIFFIYSNPFIKESVLLSTSTG---ERFNRRTFKVADVPYIQTKSYASTDLVLLAEHEP-----FYYTLEKDVIKEHPLSDPFAFWY---------------EGKDVTIEAYNVDTTGNEIHIN-DRKTKKEYTLTLPP-LVTMGASDENFIYIIQSMSIYVIDRKTEEMIETLSLASYADQFADSEEFIVASSDHKLTVIEKGTWKTTYIAYPEDLEYADTVYYDK-ESGSFYVAYEDKEGGANLLEYGEDFSIHTYSLKFPYMEAKFKGNLLYIVAQEEHKKG-IGGYVGVFDIHSKEMLYQFDLPEEQVKVQDFVVVDNK 4RAA Chain:A ((9-362)) ---TIISFKEHTDVNADSILELSFLKLQTKDSCLVKNVGLIRELNNCLLILDSA---NSNLYVFNKSGAFVNQIGQKGSGPGEYILLSSFFVDNNKNYIAAIDIAQDKVLYYNATDFSFLYERRLPFSTSCCLQLEDGNLLWNSREYTDSKLSDFYFVVTDSLFDIIDYKMNKEFKSGYTTGPSQMIYKVG--TNVFAYTPFDLTIYRVGTSEIVPAHSFSFEGTDIP---SLDFLNKTSNQGNSNYLYDLIQSDYISYYCVEETERDLFVCYMKNKEKYIGLYDKNTDRTYNYPIKIFQDQLKVGELNYFSIGSVDDYHVAPLDVLSLKDMAGNGYVFDDKLSELLT---ISNEEDNSILLFVRIKK-

General information: TITO was launched using: RESULT: Template: 4RAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1610 -156940 -97.48 -482.89 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -97.48 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_4RAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RAA-query.scw PDB file : Tito_Scwrl_4RAA.pdb: