Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKHISMLFVFLMAVMVLSACNSSESSSNSEVSSSKTRTVKHAMGTSDNIPANPKRIVVLTN-EGTEALLALGIKPVGAVKSWKGD--PWYD---YLKD-D--MKGVKNVGLETEPNVEAIAELKPDLIIGNKVRQEKIYDQLNAIAPTVFAESLAGNWKDNLTLYANAVNKADKGKEVIADFDKRVSDLKNKLGDQTNKTVSVVRFLSGESRIYYTDSFPGIILDQLGFKRPEKQVELFKKQKDQFTFSTDSKESIPDMDADVLFYFTYKADNAKENEKWANQWT-SSSLW-KNLKAVKSGNAHEVDDVVWTTAGGIKAANYLLDDIETYFLKTK 3BE6 Chain:A ((2-291)) -----------------------------------PVQVFTDDLGRKVTVPAHPKRIVSLHDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGT-ADIDIEAIVAAKPDLIITEPTR-NTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAILERRYQAQINALKATLDS-QKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIESI-----PEGGRMDVSAERLPELDADFVFATWRGDTGG-KPQDELATMEKVMPGWCQFLTACRSGRYVLISREEAIS-NSFASLGLMAAQIQSQIAGR-

General information: TITO was launched using: RESULT: Template: 3BE6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1461 -134365 -91.97 -481.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -91.97 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_3BE6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BE6-query.scw PDB file : Tito_Scwrl_3BE6.pdb: