Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKTKLVTDRIRLRCMED-RDQATLFGLLFNDPDVMTYYSG--LKDKRQTREWVNWNQRNEKGYGVSLWIAEDKRTGEFLGQCGIVPQQIENQTVMEIGYMFARRHWGNGYAQEAARACLDYGFNERQFGKMAALIDPGNKASIRVAEKIGMHYSKTIRKW----N--KPIAVYERKSYN 2ZW5 Chain:A ((9-174)) ---TAHLRTARLELTPLDPAADARHLHH-AYGDEEVMRWWTRPACADPAETERYLTSCAAA---PGARLWTIRAP-DGTVPGMAGLLGGT----DVPGLTWLLRRDSWGHGYATEAAAAVVGHALEDGGLDRVEAWIEAGNRRSLAVAARVGLTERARLAQHYPHRPGPHEMVVLGKA---

General information: TITO was launched using: RESULT: Template: 2ZW5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 818 -86498 -105.74 -550.94 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -105.74 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_2ZW5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZW5-query.scw PDB file : Tito_Scwrl_2ZW5.pdb: