Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKLKIGITCY---P---SVGGSGIIATELGKQLAEKGHEIHFITSSIPFRLNT---YHPNIHFHEVEVNQYAVFK---YPPYDLTLASKIAEV--AERENLDIIHAHYALPHAVCAYLAKQMLKRNIGIVTTLHGTDITVLGY----DPSLKDLIRFAIESSDRVTAVSSALAAETYDLIK-PEKKIETIYNFIDERVYLKKNT-AAIKEKHGILPDEKVVIHVSNFRKVKRVQDVIRVFRNIAG-K--TKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQD--RVEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTNIGGIPEVIKNNVSGFLVDVGDVTAATARAMSILEDEQLSNRFTKAAIEMLENEFSSKKIVSQYEQIYADLAEPE 3C48 Chain:A ((19-428)) -SHMRVAMISMHTSPLQQ---GMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVAENLRVINIAAGPYEGLSKEELPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQ-VGWLLRDL--WRIPLIHTAHTLAAVKNSYDTPESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYDADPDRISVVSPGADVELYSPG--TERSRRELGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICGGPS--DTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDAVEHA-RTFSWAATAAQLSSLYNDAIAN-

General information: TITO was launched using: RESULT: Template: 3C48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1949 -3905 -2.00 -10.73 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -2.00 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_3C48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C48-query.scw PDB file : Tito_Scwrl_3C48.pdb: