Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAINKAVSIDTAFQTIMQELRERSARFLGTKQAVSEEKAEFDCPYCKDRGIVVYRVHKDTSWHLDEQLDLMVPDDMVSEDDFLLGKVCTPDKASEWKDTYSKQCECVRRKKIARLMAA-------SGITEEFEKLLFGNFITDGKPDMIKDAYECAVEYYKDFQKIKGERQNSIALLGQPGSGKTHLLTAIMN------------------NLIKKKSVHCMYFPYVEGMGDLK---------ANFDNLEAKLDAMRKVEVL----------------FIDDLFKPINGQPR-----------ATDWQVEQIQSVLNYRYLNHKPLLISSELTIDEILDIDEALGSRIHQMCRDY---IVIIRGDRMQLNHRLGD--WE 1N25 Chain:A ((7-368)) ----KQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEM--CLKCIKKE---QPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVY--------NIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLD

General information: TITO was launched using: RESULT: Template: 1N25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1214 -5354 -4.41 -18.09 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -4.41 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_1N25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N25-query.scw PDB file : Tito_Scwrl_1N25.pdb: