Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNILLVGMLVLLLMFVSACSGTASKGSSSDSASEKTEMRTYKSPKGNVNIPAHPKRIVTDF--YAGELLSVGA--NVVGSGSWSFDNPFLKSKLKNVKDVGD--PISVEKVMELQPDLIVVMNE--ENVDKLKKIAPTVVIPY---NTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFWVFNDNGGRGGQAVYNALGLKAPEKIEQDVIKKGEMKQLSQEVIPEYAADYMFITDYNPK-GESKTLDKLENSSIWKNLDAVKHNRVFINDFDSFYPYDPISVSKQVDIITDMLIKRAEENKK 5A1J Chain:A ((7-285)) ----------------------------------SDEGDSFLVKDSLGENKIPKNPSKVVILDLGILDTFDALKLNDKVVGVPAKN-L-PKYLQQFKNKPSVGGVQQVDFEAINALKPDLIIISGRQSKFYDKLKEIAPTLFVGLDNANFLSSFENNVLSVAKLYGLEKEALEKISDIKNEIEKAKSIVDE----DKKALIILTNS-NKISAFGPQS-RFGI-IHDVLGINAVDENIKV---GTHGKSINSEFILEKNPDYIFVVDRNVILGNKERAQGILDNALVAKTKAAQNKKIIYLDPEYWYLASGNGL-ESLKTMILEIKN-------

General information: TITO was launched using: RESULT: Template: 5A1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1399 73549 52.57 275.46 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 52.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_5A1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A1J-query.scw PDB file : Tito_Scwrl_5A1J.pdb: