Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGF---------FP----EPAILNKELKLRNLRIAGKWFSSFILRDG---LGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTGVQT----------AEEV-DRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFTVPGDGCIDFREVYQLLLKHSYSGWI-VIEAEQDPDVANPLEYALIARNYIDQQLLDLA 3OBE Chain:A ((19-303)) ---AGKKMGLQTYSLGQEL------LQDMPNGLNRLAKAGYTDLEIFGYREDTGKFGDY-TTFIASKDYKKMVDDAGLRISSSHLTPSLREYTKENMPKFDEFWKKATDIHAELGVSCMVQPSLP--------------RIENEDDAKVVSEIFNRAGEITKKAGILWGYHNHSNEFKRVLKAGEKPEPKGTYIEELFLKNTDPDKVMFELDVYWAVMGQQDPVEWMENYPNRFKLLHIKDRWI-----------------------IGDSGMMNFPNIFKKAYEIGILGYYVELEGDKKGR--TQFEGVEKSAAYLQAAPF---

General information: TITO was launched using: RESULT: Template: 3OBE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1231 9112 7.40 37.19 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 7.40 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_3OBE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OBE-query.scw PDB file : Tito_Scwrl_3OBE.pdb: