Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIHTLTQFEAVFLGAVGNPKL--VPDHISLWGLLLKIRRELELSINMRPAKQMAGIT--SPLLH--PNDFDFVVIRENSEGEYSEVGGRIHRGDDEIAIQNAVFTRKATERVMRFAFELAKKRRSHVTSATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDH-FGEEELGAKILDVMEQVTADGIKTRDIGGQSTTAEVTDEICSRLRKL 4WUO Chain:A ((2-344)) ---ASMKVAVLPGDGIGPEVTEAALKVLRALDEAEG-LGLAYEVFPFGGAAIDAFGEPFPEPTRKGVEEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEEIARGVDVLIVRELTGGIYF---GEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVVSVDKAN-VLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASL---GRGTPVFAPVHGSAPDIAGKGIANPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLE-TPPPDLGGSAGTEAFTATVL------

General information: TITO was launched using: RESULT: Template: 4WUO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1994 -190909 -95.74 -568.18 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -95.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4WUO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WUO-query.scw PDB file : Tito_Scwrl_4WUO.pdb: