Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITKEEVNSYYQKAGIVLTDEEVDQIQLMDYGLGKERKVGLQLFVYVNTDRYCSKELVLFPGQTCPEHRHPPVDGQEGKQETFRCRYGKVYLYVEGEKTPLPKVLPPQEDREHYTVWHEIELEPGGQYTIPPNTKHWFQAGEEGAVVTEMSSTSTDKHDIFTDPRI 4E2G Chain:A ((36-118)) -----------------------------------------------IQGKNLMLNWVRIEPNTEMPAHEHPH-------EQAGVMLEGTLELTIGEET---------------------RVLRPGMAYTIPGGVRHRARTFEDGCLVLDIFSPPRED---------

General information: TITO was launched using: RESULT: Template: 4E2G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 357 -32302 -90.48 -389.18 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -90.48 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.710

(partial model without unconserved sides chains): PDB file : Tito_4E2G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E2G-query.scw PDB file : Tito_Scwrl_4E2G.pdb: