Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASRMEAMSLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELE----KQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGA---RYDLP------------------HLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV--MHFDTLYEKLDQADLLLVIGTSLEVAPAR-FVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 3RIY Chain:B ((11-256)) MADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLA-FAHNPSRVWEFYH--YRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAG-----CGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAAR--GVPVAEFNTETTPATNRF-------------------

General information: TITO was launched using: RESULT: Template: 3RIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1077 -100703 -93.50 -461.94 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -93.50 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_3RIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RIY-query.scw PDB file : Tito_Scwrl_3RIY.pdb: