Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIEHYVALVKTGCAEYGQMNEMTLTEIADCLFCTERNAKLILHKLENSNWIIRESGAGRGRKSKIAFLRRPEELLLQTAKEYTMSGKLKKAKELLQQYQSAFPGLQNEYNMWLSEVFGFVTETGENGAKDVLRLFITPESVSSLDPCQIFLRSEGH-FVKQIFDTLFTFDSDMQEPKPHLVHGWEEVG-KKQWRFFLRKGVLFHNGQ------PLTSRDVAFTFQRFLELADNPYK-------------WLLHGVKQVLEKGPYCVELILDKPNALLPYALCDERLSILPAEQG-------------GGKNGTGPFQMNQQH-SGMLVLEANERYFKGRPYLDRVEFVFSEQAGE-MN---GFTIQ-E-----------KQTCPEQQTVFDERHVQYLSLNLKKKGPLQHRSFRKALRLLISSERLVREAGGHRRIPVTSFLHPSP---------FEWEGVSPSELLKKSGYE-GETIVLYTFS-----ETDHREDAEWIQNICAQHGIRLTLQFCDAADLRRPEIVQMADIIHDSATFYQDSEFGFLHLLLSEN-----SFLYQHLSEKLTQICSGMTERMFSMPDRCSRINILRDIDRQMIQELNAIPLYQNVLQVTSSKNVKGLVLDEEGWIDLYSVWLSK 5F1Q Chain:A ((29-536)) -----------------------------------------------------------------------------------------------------------------------------------KTLVYCS-EGSPEGFNPQLFTSGTTYDASSVPIYNRLVEFKIGTTEIEPSLAERWEVSEDGKTYTFYLRKGVKWQDNKDFKPTRDFNADDVIYSFMRQKDDK-NPYHKVSGGSYEYFQGMGMGDLITNVVKVDDNTVRFELTRPESPFLADLAMDFASILSAEYADNMLKAGTPEKVDLNPIGTGPFQLQQYQKDSRILYKAFPGFWGTKPKIDRLVFSITPDASVRYAKLQKNECQIMPYPNPADIARMKEDKTINLMEQPGLNVGYLSFNIEK-KPLDNLKVRQALTMAVNKDAIIDAVYQGAGQAAKNLIPPTMWGYNDDVKDYAYDPAKAKELLKEAGLPDGFSIDLWAMPVQRPYNPNARRMAEMIQSDWAKIGVKAKIVTYEWGEYLKRAKDGEHETVMMGWTGDNGDPDNFFATLFSCDAAKQGSNYSKWC----YKPFEDLIQPARAEADHDKRVALYKQAQVVMNEQAPALIIAHSTVYEPVRKEVKGYVVDPLGKHHFDNVSLDA

General information: TITO was launched using: RESULT: Template: 5F1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2441 -176987 -72.51 -405.00 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -72.51 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_5F1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F1Q-query.scw PDB file : Tito_Scwrl_5F1Q.pdb: