Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPEGKIIKALSGFYYVLDESEDSDKVIQCRGRGIFRKNKITPLVGDYVVYQAE-NDKEGYLMEIKERTNELIRPPICNVDQAVLVFSAVQPSFSTALLDRFLVLVEANDIQPIICITKMDLIEDQDTEDTIQAYAEDYRNIGYDVYLTSSKDQDSLADIIPHFQDKTTVFAGQSGVGKSSLLNAISPELGLRTNEISEHLGRGKHTTRHVELIHTS-GGLVADTPGFSSLEFT-DIEEEELGYTFPDIREKSSSCKFRGCLHLKEPKCAVKQAVEDGELKQYRYDHYVEFMTEIKDRKPRY 2YV5 Chain:A ((7-295)) -KRGLVVDREAQMIGVYLF--EDGKTYRGIPRGKVLKK-TKIYAGDYVWGEVVDPN-TFAIEEVEERKNLLIRPKVANVDRVIIVETLKMPEFNNYLLDNMLVVYEYFKVEPVIVFNKIDLLNEEEK-KELERWISIYRDAGYDVLKVSAKTGEGIDELVDYLEGFICILAGPSGVGKSSILSRLTGE-ELRTQEV---------TTTGVRLIPFGKGSFVGDTPGFSKVEATMFVKPREVRNYFREFLR--YQCKYPDCTHTNEPGCAVKEAVKNGEISCERYKSYLKIIKVYLEEI---

General information: TITO was launched using: RESULT: Template: 2YV5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1461 -174304 -119.30 -629.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -119.30 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_2YV5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YV5-query.scw PDB file : Tito_Scwrl_2YV5.pdb: