Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVYIGNGHYCYSNSTAMFLSSIGENVSPQLVEILTGVGLGAMIEYEKNLYFSMRDPDDGINYALNILGFTAEEHQQASDLDDPFPLLKQQIKQNPVILGPLDMGELTYHPNHKNLNGSDHYVLGYQMDNENIYVQDPAGFPFVPLSLDQFKKAWM-AERIPYRKG-INKYWSTAKKVV--TLDNNEIYERAIDYFKRTYRKFEKVDIGLIGREAICFYADQLLNAPITADTIRHTTFFLFQLSARRANDYAMYFKDRHSHLSVLKTEQAKVFGICHSMSVNKDWKGISEKLMKLADLEDNFRLELLKVGY 2GSV Chain:A ((3-69)) -------------------------------------------------------------------------------------------------------------------------------------------ELFSVPYFIENLKQHIEMNQSEDKIHAMNSYYRSVVSTLVQDQLTKNAVVLKRIQHLDEAYNKVKRG---------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 150 -4077 -27.18 -64.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -27.18 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.690

(partial model without unconserved sides chains): PDB file : Tito_2GSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GSV-query.scw PDB file : Tito_Scwrl_2GSV.pdb: