Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFEKMKQWMEFAQQMYGGDFWKQVFDEDQKTPFMTNGQSPFPFAQQDQRGKGDASFPSMDIVDTVAEVQFLIYLPGYRKQDVHILSYGDYLVVKGQRFSYFNEQDFRQKEGKYGSFEKKIPLSDHLHG--KMNAIFKDGILYITIQKDEGQAKTIVIDD 3IGF Chain:A ((297-369)) --------------------------------------------------------PKPITID--THNRQVRLFLPGFDKKQVKLTQYGPEVTVEA------------------GDQRRNIFLPPALSGRPITGAKFQNNYLIISFLEH-----------

General information: TITO was launched using: RESULT: Template: 3IGF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 271 -40505 -149.46 -570.49 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -149.46 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.707

(partial model without unconserved sides chains): PDB file : Tito_3IGF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IGF-query.scw PDB file : Tito_Scwrl_3IGF.pdb: