Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKAIIKEDVQASLERYADRPVYIHLETTTGSYSAHLNEKNMTVVAYIRNAKVTYHQAKIKGNGPYRVGLKTEEGWIYAEGLTEYTVDEENRLLMAGHLPGGKLAISLQISEKPFTV 2CBL Chain:A ((218-305)) THPGYMAFLTYDEVKARLQKFIHKP---------GSYIFRLSCTRL---------------------GQWAIGYVTADGNILQTIPHN---KPLFQALIDGFREGFYLFPDGRNQNPDLTG

General information: TITO was launched using: RESULT: Template: 2CBL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 323 21204 65.65 255.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 65.65 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_2CBL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CBL-query.scw PDB file : Tito_Scwrl_2CBL.pdb: