Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 1ZS6 Chain:D ((22-168)) --ERTFLAVKPDGVQRRLVGEIVRRFERKGFKLVALKLVQASEELLREHYAELRERPFYGRLVKYMASGPVVAMVWQGLDVVRTSRALIGATNPADAPPGTIRGDFCIEVGKNLIHGSDSVESARREIALWFRADELLCWEDSAGHWLY

General information: TITO was launched using: RESULT: Template: 1ZS6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 664 -73262 -110.33 -498.38 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.91 3D Compatibility (PKB) : -110.33 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_1ZS6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZS6-query.scw PDB file : Tito_Scwrl_1ZS6.pdb: