Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYGVYMDRPLSAGEEDRMMAAVSAE---KREKCRRFYHKEDAHRT----LIGDMLIRTAAAKAYGLDP------AGISFGVQEYGKPY----------IPALPDMHFNISHSGRWIVCAVDSKPIGIDIEKMKPGTIDIAKRFFSPTEY----------------SDLQAKHPDQQTDYF--YHLWSMK-----ESFIKQAGKGLSLP---LDSFSVRLKDDGHVS----IELPDGHEPCFIRTYDADEEYKLAVCAAHPDFCDGIEMKTYEELL-------- 4KXR Chain:C ((8-298)) -------TDITVNVDGFWMLQALLDIRHVAPELRCRPYVHPGMAVMREQGIVVNDAVNEQVAARMKVLAAPDLEVVALLSRGKLLYGVIDDENQPPGSRDIPDNEFRVVLARRGQHWVSAVRVGNDITVDDVTVSD-SASIAALVMDGLESIHHADPAAINAVNVPMEEMLEATKSWQESGFNVFSGGDLRRMGISAATVAALGQALSDPAAEVAVYARQYRDDAKGPSASVLSLKDGSGG-RIALYQQAREAWLAICPATPQLVQVGVKTVLDTLPYGEWKTHS

General information: TITO was launched using: RESULT: Template: 4KXR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 875 -7317 -8.36 -34.03 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -8.36 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.129

(partial model without unconserved sides chains): PDB file : Tito_4KXR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KXR-query.scw PDB file : Tito_Scwrl_4KXR.pdb: