Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTHLQAKATLHNGVEMPWFGLGVFQVEEGSELVNAVKTAIVHGYRSIDTAAIYGNEAGVGEGIREGIEEAGISREDLFITSKVWNADLGYEETLAAFETSLSKLGLDYLDLYLIHWP------------------VEGK--YKEAWRALETLYKEGRIKAIGVSNFQIHHLEDLMTAA--EIKPMINQVEFHPRLTQKELIRYCQNQGIQMEAWSPL----------MQGQLLDHPVLADIAQTYNKSVAQIILRWDLQHGIITIPKSTKEHRIKENASVFDFELTQDDMNRIDALNENLRVGPDPDNFDF 4JII Chain:X ((1-299)) --SHMATFVELSTKAKMPIVGLGTWKSPLG-KVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWR----------

General information: TITO was launched using: RESULT: Template: 4JII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1375 -47507 -34.55 -177.93 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain X : 0.79 3D Compatibility (PKB) : -34.55 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4JII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JII-query.scw PDB file : Tito_Scwrl_4JII.pdb: