Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLS-AALL---MKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALKEMGSIEA------------------------------------DGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLYEEHERIYNAVAAGNGAQAEAAMLAHLTNVEDVLSGYFEENVQ 4P96 Chain:A ((19-268)) SIWNGRFPPGSILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNQFMETSGLHILDTLMTLDAENATSIVEDLLAARTNISPIFMRYAFKLNKESAERIMINVIESCEALVNAPSWDAFIAASPYAEKIQQHVKEDSEKDELKRQEILIAKTFNFYDYMLFQRLAFHSGNQIYGLIFNGLKKLYDRVGS-Y--YFS-N-PQARELAMEFYRQLLAVCQSGEREHLPQVIRQYGIASGHIWNQMKM----

General information: TITO was launched using: RESULT: Template: 4P96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 702 -51204 -72.94 -243.83 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -72.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_4P96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P96-query.scw PDB file : Tito_Scwrl_4P96.pdb: