Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDN-VSISDFEYLDLNKTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYY-DMLNMLQNKQVIQLGNRASRHTIT--TSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDNGV--LTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGRDIVHPTKDAEQHRKFMEYEQHVIDEFKDLIPIIPYERKRAANCGAGWKSIVISPFGEVRPCALFPKE--FSLGNIFHDSYESIFNS----PLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI-------------------- 4X8E Chain:A ((8-435)) PHRAELARQLIDARNRTLRLVDFDDAELRRQYDPLMSPLVWDLAHIGQQEELWLLRGGDPRRPGLLEPAVEQLYDAFVHPRASRVHLPLLSPAQARRFCATVRSAVLDALDRLPEDADTFAFGMVVSHEHQHDETMLQAL-NLRSGEPLLGS-------GTALPPGRPGVAGTSVLVPGGPFVLGVDLAD----------------EPYALDNERPAHVVDVPA---FRIGRVPVTNAEWRAFID-DGGYRQ----RRWWSDAGWAYRCEAGLTAPQFWNPDG--TRTR--------------FGHVEDI--PPDEPVQHVTYFEAEAYAAWAGARLPTEIEWEKACAWDPATGRRRR--YPWGDAAPTAALANLGGDALRPAPVGAYPAGASACGAEQMLGDVWEWTSSPLRPWPGFTPMIYQRYSQPFF-EGAGSGDYRVLRGGSWAVAADILRPSFRNWDHPIRRQIFAGVRLAWDVD

General information: TITO was launched using: RESULT: Template: 4X8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1956 10905 5.57 27.61 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 5.57 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.078

(partial model without unconserved sides chains): PDB file : Tito_4X8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X8E-query.scw PDB file : Tito_Scwrl_4X8E.pdb: