Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLLAIYPGSIVSEELCIREGCLCFFDESNQRYISIPKTEISEKEVLVLQSFLTPADEGNQLSMKSPEENKWFSFLFSRGELPAYIKKRTRFVHFHLFGKIERTSFTEAVRHFWPVSFVIVWIHEDRGVIV---EQESEAAAEKDELESLAKVLESDFYFSVRFYAGRFYEPDECLRKHYAREQAYFLFAEKRLPQVQ-SVTFEMIFPFLLLETEKEKLETLLSEEAELLFGSESELRKTIKLFIENNSNV------TLTAKKLHLHRNSLQYRIDKFIERSGIDIKSYKGALLAYFICLQNESSE 1EFV Chain:B ((4-255)) LRVLVAVK--RVIDYAVKIR-----VKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKL-----------------------------VKEVIAVSCGP---AQCQETIRTALAMGA-------DRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKV-DLVLLGKQAIDDD-------CNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTS-KLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI

General information: TITO was launched using: RESULT: Template: 1EFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1248 -40650 -32.57 -167.97 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -32.57 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_1EFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFV-query.scw PDB file : Tito_Scwrl_1EFV.pdb: