Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIRTENFQTFIRLYPVVTFILALQAVLWLFFSLPAHSVVLWRDTVTGYNLGVANGEWWRLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIGNIGTYVTEPLDYVHVGA-SGAIFGLFGVYLFMVLFRNELIGQEHSKMIITLLAFAVL---------MSFINSNINMMAHLFGLCGGFLLSFLCVQKKERRY 5F5D Chain:A ((134-272)) --------------------------------------------------------EFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSG---YVQQKFSGPWFGGLSGVVFALMGY----VWLRGERDPQSGIYLQRGLIIFALIWIVAGWFDL-----------AHIAGLAVGLAMAFVDSLNA----

General information: TITO was launched using: RESULT: Template: 5F5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 487 -78930 -162.07 -652.31 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -162.07 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_5F5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F5D-query.scw PDB file : Tito_Scwrl_5F5D.pdb: