Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVRKSFVLLLTGLLAVLILSACGQKTQQDIVAGLDEKAKEYTSYKAKAKMTIETGSEPQVYNVEIWHKKPSLYRVYLENPKKDQNQVILRNENGVFVLTPSLNKSFRFQSDWPNNSSQVYLFESLVKDVQNDSDAVFTAKEKKYVFETKTNYQHNKMLPTQEITFNKKDMSPSSVKVMDTDRKVMVKVEFSSFEFNKQFDKESFDEKKNMTLSQMDVATSAKPSDTFAV---------------KTPLEL---PLGVKLLEEK-----DISTEDGKRIIMTYGGEKSFTLIQEKAQIAKASSSVTLNGEPVNLGYTIGALSDASLSWTYDGVDYLLSSKDLSKEEMVTVAKSMQGQSSK 1E4F Chain:T ((138-283)) --------------------------------------------------------------------------------------------------------------------------------------------------------------LLDDERIVFNPLDMKASKIAIEYTSIVVPLKVYEMFYNFLQDTVKSPFQLKSSLVSTAEGVLTTPEKDRGVVVVNLGYNFTGLIAYKNGVPIKISYVPVGMKHVIKDVSAVLDTSFEESERLIITHGNAVYNDLKEEEIQ---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1E4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 171 -9576 -56.00 -81.85 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain T : 0.57 3D Compatibility (PKB) : -56.00 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.102

(partial model without unconserved sides chains): PDB file : Tito_1E4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4F-query.scw PDB file : Tito_Scwrl_1E4F.pdb: