Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINVDVKQNENDIQVNIAGEIDVYSAPVLREKLVPLAEQGADLR---ICLKDVSYMDSTGLGVFVGTFKMVKKQGGSLKLENLSERLIRLFDITGLKDIIDISAKSEGGVQ 1BUZ Chain:A ((2-99)) --LGIDMNVKESVLCIRLTGELDHHTAETLKQKVTQSLEKD-DIRHIVLNLEDLSFMDSSGLGVILGRYKQIKQIGGEMVVCAISPAVKRLFDMSGLFKII-----------

General information: TITO was launched using: RESULT: Template: 1BUZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 352 -45306 -128.71 -476.91 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -128.71 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_1BUZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BUZ-query.scw PDB file : Tito_Scwrl_1BUZ.pdb: