Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSMILAVFIFLLTLVLVIWQPKNLSIGWSACGGAVLALIAGVVNFHDVLTVTGIVWNATLAFVAIILISLILDNIGFFEWAALHMAKAAKGYGVRMFVYVSLLGAIVAALFANDGAALILTPIVLAMVRALHFNEKLVFPFIIASGFIADTTSLPFVVSNLVNIVSA----DYFHI-------TFIDYASRMVVPYLFSLLASIIVLYLFFRKSIPKR--YDLT-EV-KKPVEAIKDQNMFRLSWYILGLLLIGYFASEFF------------SIP-VSVVAGSIAIIFLIAA----QKSP-AVHTKKVVKEAPW------AIVFFSIGMYVVVYGVRNAGLTDV-LSDVIQAA-ADQ-GLFAGTIGMGFIAAILSSIM-NNLPTVMIDALAIAGTDT-HGMMREALIYANVIGSDLGPKITPIGSLATLLWLHVLSHKGMKISWGTYFKTGIILTIPTLLITLVGLYIWLLIIHSCF 4R0C Chain:A ((82-484)) ---------------------------------------------------------PLG-VVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGAL--V-FTVAFAGVLSSLT-VDAGYVVLIPLAGLVFQLAGRPPIAG---IATAFAAVSGGFSANLLVGPVDATLAGLSTEAAHIIDPDRTVAATGNYWFIIASTFLVTGLVTLITR--TLTEPRLAHANTVADASVDAPQIHSRAMKWTGLTLAILLAGLALLVL-PNDAPLRHPDTGSVLGSPFIHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLGISPEASQAAYRVGDSSTNIITPLMPY-FVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWSVLLGVWI-------

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1755 -274868 -156.62 -765.65 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -156.62 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: