Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYQINIANIKINGVTQNGNIDVGPTVHNSHTANSKYFGANFSLGDLSPTSSLLNTGNIDSDVSDQDQIGNPSAPISNQI 1P4Q Chain:A ((8-20)) -------------------------------------------------------SNVIDTDFIDEEV------------

General information: TITO was launched using: RESULT: Template: 1P4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3 113 37.50 8.65 target 2D structure prediction score : 1.00 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 37.50 2D Compatibility (Sec. Struct. Predict.) : 1.00 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.899

(partial model without unconserved sides chains): PDB file : Tito_1P4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P4Q-query.scw PDB file : Tito_Scwrl_1P4Q.pdb: