Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTVLILNFPAEGHVNPTLGITKAFSDKGYDVHYISTEKYKKRLEAAGATVHLHRDLLR---TTPIHVGSPNGILDFVKIHIKTSLDILQIVKDLSKSIQFDFVYYD--KFGAGELVRDYLDIPGVSSSASFLFGE-------EHLKILPLHPESGAPLELDQEC---EDLLAKMKETYGVAPKNLVQFMNNKGELNVVYTSRYFQPESDRFGDEC-LFIGPSFPKRAEKTDFPIEQLKDEKVIYISMGTVLDHTEDFFNLCIDAFSGFNG-KVVIAAGEKADLTKLKQAPENFIIAPYVPQLEVLEQSDVFITHGGMNSVNEGIHFSVPLVVMPHDKDQPMVAQRLSELHAGYVISKDEVNAQILKQAVDEVLRNDQYTAGIKKINQSFKECMDMEEVMERIDELIRQKNK 4M83 Chain:A ((10-394)) ---IAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIPPVFADKVAATGARPVLYHSTLPGPDADPEAWGS--TLLDNVEPFLNDAIQALPQLADAYADDIPDLVLHDITSYPARVLARRW-GVPAVSLSPNLVAWKGYEEEVAEPMWREPRQTERGRAYYARFEAWLKENGITEHPDTFASHPPR-----------SLVLIPKALQPHADRVDEDVYTFVGACQGDRAEEGGW-QRPAGAEKVVLVSLGSAFTKQPAFYRECVRAFGNLPGWHLVLQIGRKVTPAELGELPDNVEVHDWVPQLAILRQADLFVTHAGAGGSQEGLATATPMIAVPQAVDQFGNADMLQGLGVARKLATEEATADLLRETALALVDDPEVARRLRRIQAE----MAQEGGTRRAADLI-----

General information: TITO was launched using: RESULT: Template: 4M83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1931 -206737 -107.06 -561.78 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -107.06 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4M83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M83-query.scw PDB file : Tito_Scwrl_4M83.pdb: