Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILAIDTSNYTLGIALLREDTVIAEYITYLKKNHSVRAMPAVHSLLNDCDMAPQDLSKIVVAKGPGSYTGVRIGVTLAKTLAWSLDIPISAVSSLETLAANGRHFDGL--ISPIFDARRGQVYTGLYQY-KNGLLEQVVPDQNVMLADWLEMLKEKDRPVLFLGHDTSLHKQMIEDVLGTKGFIGTAAQHNPRPSELAFLGKEKEAADVHGLVPDYLRLAEAEAKWIESQK 4YDU Chain:C ((1-134)) MRILAIDTATEACSVALWNDGTVNAHF-ELCPREHTQRILPMVQDILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGAWRKNGATRVLAAIDARMGEVYWAEYQRDENGI-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4YDU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 582 -91771 -157.68 -700.54 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : -157.68 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_4YDU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YDU-query.scw PDB file : Tito_Scwrl_4YDU.pdb: