Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYIIL--EDQTR--LYYETHGSGTPILFIHGVLMSGQFFHKQ-FSVLSANYQCIRLDLRGHGESDKVLHGHTISQYARDIREFLNAMELDHVVLAGWSMGAFVVWDYLNQFGNDNIQAAVIIDQ-----SASDYQWEGWEHGPFDFDGLKTAMHAIQTDPLPFYESFI-------QNMFAEPPAE-TETEWMLAEILKQPAAISSTILFNQTAADYRGTLQNINVPALLCFGE-DKKFFSTAAGEHLRSNIPNATLVTFPKSSHCPFLEEPDAFNSTLLSFLDGVIGKS 4IVJ Chain:A ((1-275)) -PFITVGQENSTSIDLYYEDHGTGTPVVLIHGFPLSGHSWERQSAALLDAGYRVITYDRRGFGQSSQPTTGYDYDTFAADLNTVLETLDLQDAVLVGFSMGTGEVARYVSSYGTARIAAVAFLASLEPFLLKTDDNPDGAAPQEF-FDGI---VAAVKADRYAFYTGFFNDFYNLDENLGTRISEEAVRNSWNTAASGGFFAAAAAPTTW---YTDFRADIPRIDVPALILHGTGDRTLPIENTARVFHKALPSAEYVEVEGAPHGLLWTHAEEVNTALLAFL-------

General information: TITO was launched using: RESULT: Template: 4IVJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1424 -196227 -137.80 -766.51 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -137.80 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_4IVJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IVJ-query.scw PDB file : Tito_Scwrl_4IVJ.pdb: