Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSKLIFFDIDGTIYDHDKN-IPESTRKTVAELQRQGHHVFIASGRSPFLVKPILEELG----IHSFISYNGQFVVFENQVIYKNPLPENAIRRLLKQADEGKHPVVFMAEDTMKATVADHPHVLEGIGSLKTDYPETDDL----FYEG--KEIFQLLLFCQDEEEK-AYAAFPEFDLVRWHELSTDVLPHGGSKAEGIKKVIERLPFDIGDTYAFGDGLNDLQMIEYVGTGVAMGNAVPELKEIADFVTKPVDEDGIAYAVKELGLLK 2RBK Chain:A ((2-261)) --TKALFFDIDGTLVSFETHRIPSSTIEALEAAHAKGLKIFIATGRPKAIINN-LSELQDRNLIDGYITMNGAYCFVGEEVIYKSAIPQEEVKAMAAFCEKKGVPCIFVEEHNISVC---QPN--EMVKKIFYDFLHVNVIPTVSFEEASNKEVIQMTPFITEEEEKEVLPSIPTCEIGRWYPAFADVTAKGDTKQKGIDEIIRHFGIKLEETMSFGDGGNDISMLRHAAIGVAMGQAKEDVKAAADYVTAPIDEDGISKAMKHFGII-

General information: TITO was launched using: RESULT: Template: 2RBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1518 -170373 -112.24 -686.99 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -112.24 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_2RBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RBK-query.scw PDB file : Tito_Scwrl_2RBK.pdb: