Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPGQDREKIVNIEERIPKIKEQRKQKANRRLISFIMLFFIMVLIIVYLQTPISKVSTISVTGNE-NVSKKEIIDLSD-INSGDTEFWSLDKQKTEKKIQQNKLVKKAEISKSLPNKINIAIEEYKAIAYLEKDDVYYEVLENGSVLPNEVT--PDDAGPILVNWTNAK-KRSQMAKQLDALSNSLKQSISEIYYTPVKMDENRIKLYMNDGYVVTASIKTFADRMKTYPSIISQLS--SNKKGIIHLEVATYFEEFGKNDKAAKKEDEN 2VH2 Chain:A ((57-246)) ------------------------------------------------------PLSKLVVTGERHYTTNDDIRQAILSLGAPG-TFMTQDVNIIQQQIERLPWIQQASVRKQWPDELKIHLVEYVPFARWN---DLHMVDEQGRSFSVPSERVGKQKLPLLYGPEGSEQDVLEGYRAINKVLAANKYQLKMVAMS----ARHSWQLALDNDVRLELGRDDRMGRLQRFIELYPMLQQPDKRVSYVDLRYE-------------------

General information: TITO was launched using: RESULT: Template: 2VH2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 727 -64697 -88.99 -355.48 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -88.99 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_2VH2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VH2-query.scw PDB file : Tito_Scwrl_2VH2.pdb: