Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRRTAREKALQALFQIDVS-DIAVNEAIEHALDE---EKTDPFFEQLVHGVLEHQDQLDEMISKHLVNWKLDRIANVDRAILRLAAYEMAYAEDIPVNVSMNEAIELAKRFGDDKATKFVNGVLSNIKSDIEQS 1TZW Chain:A ((5-130)) --RRRMRLAVFKALFQHEFRRDEDLEQILEEILDETYDKKAKEDARRYIRGIKENLSMIDDLISRYLEKWSLNRLSVVDRNVLRLATYELLFEKDIPIEVTIDEAIEIAKRYGTENSGKFVNGILDRI-------

General information: TITO was launched using: RESULT: Template: 1TZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 -23578 -50.70 -193.26 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -50.70 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_1TZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TZW-query.scw PDB file : Tito_Scwrl_1TZW.pdb: