Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEADMLFSVTVPGSTANLGPGFDSVGMALSR-YLKLTVFESDKWSFEAETETVAGIPAGTD-NLIYQVAKRTADLY--GKEMPPVHVKVWSDIPLARGLGSSAAAIVAAIELADELCGLKLSEADKLHLASLEE------GHPDNAGASLVGGLVIGLHEDDETQMIRVP-NADIDVVVVIPFYEVLTRDARDVLPKEFPYADAVKASAVSNILIAAIMSKDWPLVGKIMKKDMFHQPYRAMLVPELSKVEHVAEMKGAYGTALSGAGPTILVMTEKGKGEELKEQLALHFPHCEVDALTVPKEGSIIERNPLYQVKSV 1FWL Chain:A ((5-287)) -----MKVRVKAPCTSANLGVGFDVFGLCLKEPYDVIEVEAIDDKEIIIEVDD-KNIPTDPDKNVAGIVAKKMIDDFNIGK---GVKITIKKGVKAGSGLGSSAASSAGTAYAINELFKLNLDKLKLVDYASYGELASSGAKHADNVAPAIFGGFTM-VTNYEPLEVLHIPIDFKLDILIAIPNISINTKEAREILPKAVGLKDLVNNVGKACGMVYALYNKDKSLFGRYMMSDKVIEPVRGKLIPNYFKIKEEVKDK-VYGITISGSGPSIIAFPKEEFIDEVENILRDYYEN--------------------------

General information: TITO was launched using: RESULT: Template: 1FWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1572 29798 18.96 109.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 18.96 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_1FWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWL-query.scw PDB file : Tito_Scwrl_1FWL.pdb: