Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRNKTLWILRDYVYILIGAAITAVSFNVFLLPNKIAAGGVSGISTILQSYGFEAAYVQWIINIPLFIAGVILLGGKFGLKTLAGSVFLPLVVFLTRDIQPATHHELLAAIFGGVGIGIGIGIVYLGKGSTGGTALAAQIIHKYSGLSLGKCLAIIDGMIVVTAMIVFNIEQGLYAMLGVYVSSKTIDVVQVGFNRSKMALIITKQ-EQAVKEAVLQKIDRGVTKISAVGGYTDDDRPILMCVVGQTEFTKLKQIVKQIDESAFVIVADASEVLGEGFKRA 4RLE Chain:A ((11-75)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LIVAVVQDQDSNRLLKTLTD-HNFRVTKLATTGGFLKSGNTTFMIGVEDIRVNKALSLIKENGQKR------------------

General information: TITO was launched using: RESULT: Template: 4RLE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 209 -4251 -20.34 -66.41 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.45 3D Compatibility (PKB) : -20.34 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.45 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_4RLE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RLE-query.scw PDB file : Tito_Scwrl_4RLE.pdb: